p rip1 antibody  (Cell Signaling Technology Inc)

Journal: Journal of Animal Science and Biotechnology

Article Title: Necroptosis contributes to deoxynivalenol-induced liver injury and inflammation in weaned piglets

doi: 10.1186/s40104-024-01117-1

Figure Lengend Snippet: Effects of dietary or gavage with DON on protein expression of necroptosis signals in liver cells of piglets. A The protein expression of necroptosis signals after dietary DON exposure. B Representative bands of necroptosis signals after dietary DON exposure. C The protein expression of necroptosis signals after DON gavage. D Representative bands of necroptosis signals after DON gavage. Values are means ± SE, n = 6. a,b Values without a common letter differ significantly ( P < 0.05). DON, Deoxynivalenol; p-MLKL, Phosphorylated mixed lineage kinase domain-like protein; p-RIP1, Phosphorylated receptor interacting protein kinase 1; p-RIP3, Phosphorylated receptor interacting protein kinase 3; t-MLKL, Total mixed lineage kinase domain-like protein; t-RIP1, Total receptor interacting protein kinase 1; t-RIP3, Total receptor interacting protein kinase 3

Article Snippet: Specific primary antibodies including mouse anti-t-RIP1 (1:1,000, LifeSpan BioSciences, Seattle, Washington, USA), rabbit anti-phosphorylated RIP1 (p-RIP1) (1:2,000, Cell Signaling Technology, Boston, Massachusetts, USA), mouse anti-t-RIP3 (1:1,000, Santa Cruz Biotechnology, Santa Cruz, CA, USA), rabbit anti-phosphorylated RIP3 (p-RIP3) (1:2,000, Cell Signaling Technology, Boston, Massachusetts, USA), rabbit anti-t-MLKL (1:1,000, Cell Signaling Technology, Boston, Massachusetts, USA), rabbit anti-phosphorylated MLKL (p-MLKL) (1:1,000, Cell Signaling Technology, Boston, Massachusetts, USA) and mouse anti-β-actin (1:10,000, Sigma Aldrich, St. Louis, Missouri, USA).

Techniques: Expressing

Journal: Journal of Animal Science and Biotechnology

Article Title: Necroptosis contributes to deoxynivalenol-induced liver injury and inflammation in weaned piglets

doi: 10.1186/s40104-024-01117-1

Figure Lengend Snippet: Effects of Nec-1 on protein expression of necroptosis signals in liver of piglets after DON gavage. Piglets were given a gavage with 2 mg/kg BW DON or an equal volume of normal saline after intraperitoneal injection of 0.5 mg/kg BW Nec-1 or an equal volume of 5% dimethylsulfoxide (DMSO). Pigs were euthanized at 6 h after DON or saline gavage. A–I Protein expression of necroptosis signals. J Representative bands. Values are means ± SE, n = 6. a,b Values without a common letter differ significantly ( P < 0.05). DON, Deoxynivalenol; p-MLKL, Phosphorylated mixed lineage kinase domain-like protein; p-RIP1, Phosphorylated receptor interacting protein kinase 1; p-RIP3, Phosphorylated receptor interacting protein kinase 3; t-MLKL, Total mixed lineage kinase-like protein; t-RIP1, Total receptor interacting protein kinase 1; t-RIP3, Total receptor interacting protein kinase 3

Article Snippet: Specific primary antibodies including mouse anti-t-RIP1 (1:1,000, LifeSpan BioSciences, Seattle, Washington, USA), rabbit anti-phosphorylated RIP1 (p-RIP1) (1:2,000, Cell Signaling Technology, Boston, Massachusetts, USA), mouse anti-t-RIP3 (1:1,000, Santa Cruz Biotechnology, Santa Cruz, CA, USA), rabbit anti-phosphorylated RIP3 (p-RIP3) (1:2,000, Cell Signaling Technology, Boston, Massachusetts, USA), rabbit anti-t-MLKL (1:1,000, Cell Signaling Technology, Boston, Massachusetts, USA), rabbit anti-phosphorylated MLKL (p-MLKL) (1:1,000, Cell Signaling Technology, Boston, Massachusetts, USA) and mouse anti-β-actin (1:10,000, Sigma Aldrich, St. Louis, Missouri, USA).

Techniques: Expressing, Saline, Injection